Nitrate reductase gene involvement in hexachlorobiphenyl dechlorination by Phanerochaete chrysosporium.
Identifieur interne : 000768 ( Main/Exploration ); précédent : 000767; suivant : 000769Nitrate reductase gene involvement in hexachlorobiphenyl dechlorination by Phanerochaete chrysosporium.
Auteurs : Supriyo De [États-Unis] ; Michael Perkins ; Sisir K. DuttaSource :
- Journal of hazardous materials [ 0304-3894 ] ; 2006.
Descripteurs français
- KwdFr :
- Alignement de séquences (MeSH), Chlore (composition chimique), Chlore (métabolisme), Chromatographie en phase liquide à haute performance (MeSH), Données de séquences moléculaires (MeSH), Nitrate reductase (génétique), Nitrate reductase (métabolisme), Phanerochaete (enzymologie), Phanerochaete (génétique), Polychlorobiphényles (composition chimique), Polychlorobiphényles (métabolisme), Régulation de l'expression des gènes codant pour des enzymes (MeSH), Régulation de l'expression des gènes fongiques (MeSH), Séquence nucléotidique (MeSH).
- MESH :
- composition chimique : Chlore, Polychlorobiphényles.
- enzymologie : Phanerochaete.
- génétique : Nitrate reductase, Phanerochaete.
- métabolisme : Chlore, Nitrate reductase, Polychlorobiphényles.
- Alignement de séquences, Chromatographie en phase liquide à haute performance, Données de séquences moléculaires, Régulation de l'expression des gènes codant pour des enzymes, Régulation de l'expression des gènes fongiques, Séquence nucléotidique.
English descriptors
- KwdEn :
- Base Sequence (MeSH), Chlorine (chemistry), Chlorine (metabolism), Chromatography, High Pressure Liquid (MeSH), Gene Expression Regulation, Enzymologic (MeSH), Gene Expression Regulation, Fungal (MeSH), Molecular Sequence Data (MeSH), Nitrate Reductase (genetics), Nitrate Reductase (metabolism), Phanerochaete (enzymology), Phanerochaete (genetics), Polychlorinated Biphenyls (chemistry), Polychlorinated Biphenyls (metabolism), Sequence Alignment (MeSH).
- MESH :
- chemical , chemistry : Chlorine, Polychlorinated Biphenyls.
- chemical , genetics : Nitrate Reductase.
- chemical , metabolism : Chlorine, Nitrate Reductase, Polychlorinated Biphenyls.
- enzymology : Phanerochaete.
- genetics : Phanerochaete.
- Base Sequence, Chromatography, High Pressure Liquid, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Fungal, Molecular Sequence Data, Sequence Alignment.
Abstract
Polychlorobiphenyl (PCB) degradation usually occurs through reductive dechlorination under anaerobic conditions and phenolic ring cleavage under aerobic conditions. In this paper, we provide evidence of nitrate reductase (NaR) mediated dechlorination of hexachlorobiphenyl (PCB-153) in Phanerochaete chrysosporium under non-ligninolytic condition and the gene involved. The NaR enzyme and its cofactor, molybdenum (Mo), were found to mediate reductive dechlorination of PCBs even in aerobic condition. Tungsten (W), a competitive inhibitor of this enzyme, was found to suppress this dechlorination. Chlorine release assay provided further evidence of this nitrate reductase mediated dechlorination. Commercially available pure NaR enzyme from Aspergillus was used to confirm these results. Through homology search using TBLASTN program, NaR gene was identified, primers were designed and the RT-PCR product was sequenced. The NaR gene was then annotated in the P. chrysosporium genome (GenBank accession no. AY700576). This is the first report regarding the presence of nitrate reductase gene in this fungus with the explanation why this fungus can dechlorinate PCBs even in aerobic condition. These fungal inoculums are used commercially as pellets in sawdust for enhanced bioremediation of PCBs at the risk of depleting soil nitrates. Hence, the addition of nitrates to the pellets will reduce this risk as well as enhance its activity.
DOI: 10.1016/j.jhazmat.2005.11.073
PubMed: 16406292
Affiliations:
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Dutta</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16406292</idno><idno type="pmid">16406292</idno><idno type="doi">10.1016/j.jhazmat.2005.11.073</idno><idno type="wicri:Area/Main/Corpus">000801</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000801</idno><idno type="wicri:Area/Main/Curation">000801</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000801</idno><idno type="wicri:Area/Main/Exploration">000801</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Nitrate reductase gene involvement in hexachlorobiphenyl dechlorination by Phanerochaete chrysosporium.</title><author><name sortKey="De, Supriyo" sort="De, Supriyo" uniqKey="De S" first="Supriyo" last="De">Supriyo De</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biology, Howard University, Washington, DC 20059, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biology, Howard University, Washington, DC 20059</wicri:regionArea><placeName><region type="state">District de Columbia</region></placeName></affiliation></author><author><name sortKey="Perkins, Michael" sort="Perkins, Michael" uniqKey="Perkins M" first="Michael" last="Perkins">Michael Perkins</name></author><author><name sortKey="Dutta, Sisir K" sort="Dutta, Sisir K" uniqKey="Dutta S" first="Sisir K" last="Dutta">Sisir K. Dutta</name></author></analytic><series><title level="j">Journal of hazardous materials</title><idno type="ISSN">0304-3894</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence (MeSH)</term><term>Chlorine (chemistry)</term><term>Chlorine (metabolism)</term><term>Chromatography, High Pressure Liquid (MeSH)</term><term>Gene Expression Regulation, Enzymologic (MeSH)</term><term>Gene Expression Regulation, Fungal (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Nitrate Reductase (genetics)</term><term>Nitrate Reductase (metabolism)</term><term>Phanerochaete (enzymology)</term><term>Phanerochaete (genetics)</term><term>Polychlorinated Biphenyls (chemistry)</term><term>Polychlorinated Biphenyls (metabolism)</term><term>Sequence Alignment (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences (MeSH)</term><term>Chlore (composition chimique)</term><term>Chlore (métabolisme)</term><term>Chromatographie en phase liquide à haute performance (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Nitrate reductase (génétique)</term><term>Nitrate reductase (métabolisme)</term><term>Phanerochaete (enzymologie)</term><term>Phanerochaete (génétique)</term><term>Polychlorobiphényles (composition chimique)</term><term>Polychlorobiphényles (métabolisme)</term><term>Régulation de l'expression des gènes codant pour des enzymes (MeSH)</term><term>Régulation de l'expression des gènes fongiques (MeSH)</term><term>Séquence nucléotidique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Chlorine</term><term>Polychlorinated Biphenyls</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nitrate Reductase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Chlorine</term><term>Nitrate Reductase</term><term>Polychlorinated Biphenyls</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Chlore</term><term>Polychlorobiphényles</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Nitrate reductase</term><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Chlore</term><term>Nitrate reductase</term><term>Polychlorobiphényles</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Chromatography, High Pressure Liquid</term><term>Gene Expression Regulation, Enzymologic</term><term>Gene Expression Regulation, Fungal</term><term>Molecular Sequence Data</term><term>Sequence Alignment</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Chromatographie en phase liquide à haute performance</term><term>Données de séquences moléculaires</term><term>Régulation de l'expression des gènes codant pour des enzymes</term><term>Régulation de l'expression des gènes fongiques</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Polychlorobiphenyl (PCB) degradation usually occurs through reductive dechlorination under anaerobic conditions and phenolic ring cleavage under aerobic conditions. In this paper, we provide evidence of nitrate reductase (NaR) mediated dechlorination of hexachlorobiphenyl (PCB-153) in Phanerochaete chrysosporium under non-ligninolytic condition and the gene involved. The NaR enzyme and its cofactor, molybdenum (Mo), were found to mediate reductive dechlorination of PCBs even in aerobic condition. Tungsten (W), a competitive inhibitor of this enzyme, was found to suppress this dechlorination. Chlorine release assay provided further evidence of this nitrate reductase mediated dechlorination. Commercially available pure NaR enzyme from Aspergillus was used to confirm these results. Through homology search using TBLASTN program, NaR gene was identified, primers were designed and the RT-PCR product was sequenced. The NaR gene was then annotated in the P. chrysosporium genome (GenBank accession no. AY700576). This is the first report regarding the presence of nitrate reductase gene in this fungus with the explanation why this fungus can dechlorinate PCBs even in aerobic condition. These fungal inoculums are used commercially as pellets in sawdust for enhanced bioremediation of PCBs at the risk of depleting soil nitrates. Hence, the addition of nitrates to the pellets will reduce this risk as well as enhance its activity.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16406292</PMID><DateCompleted><Year>2006</Year><Month>10</Month><Day>26</Day></DateCompleted><DateRevised><Year>2016</Year><Month>10</Month><Day>25</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0304-3894</ISSN><JournalIssue CitedMedium="Print"><Volume>135</Volume><Issue>1-3</Issue><PubDate><Year>2006</Year><Month>Jul</Month><Day>31</Day></PubDate></JournalIssue><Title>Journal of hazardous materials</Title><ISOAbbreviation>J Hazard Mater</ISOAbbreviation></Journal><ArticleTitle>Nitrate reductase gene involvement in hexachlorobiphenyl dechlorination by Phanerochaete chrysosporium.</ArticleTitle><Pagination><MedlinePgn>350-4</MedlinePgn></Pagination><Abstract><AbstractText>Polychlorobiphenyl (PCB) degradation usually occurs through reductive dechlorination under anaerobic conditions and phenolic ring cleavage under aerobic conditions. In this paper, we provide evidence of nitrate reductase (NaR) mediated dechlorination of hexachlorobiphenyl (PCB-153) in Phanerochaete chrysosporium under non-ligninolytic condition and the gene involved. The NaR enzyme and its cofactor, molybdenum (Mo), were found to mediate reductive dechlorination of PCBs even in aerobic condition. Tungsten (W), a competitive inhibitor of this enzyme, was found to suppress this dechlorination. Chlorine release assay provided further evidence of this nitrate reductase mediated dechlorination. Commercially available pure NaR enzyme from Aspergillus was used to confirm these results. Through homology search using TBLASTN program, NaR gene was identified, primers were designed and the RT-PCR product was sequenced. The NaR gene was then annotated in the P. chrysosporium genome (GenBank accession no. AY700576). This is the first report regarding the presence of nitrate reductase gene in this fungus with the explanation why this fungus can dechlorinate PCBs even in aerobic condition. These fungal inoculums are used commercially as pellets in sawdust for enhanced bioremediation of PCBs at the risk of depleting soil nitrates. Hence, the addition of nitrates to the pellets will reduce this risk as well as enhance its activity.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>De</LastName><ForeName>Supriyo</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Biology, Howard University, Washington, DC 20059, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Perkins</LastName><ForeName>Michael</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Dutta</LastName><ForeName>Sisir K</ForeName><Initials>SK</Initials></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>AY700576</AccessionNumber></AccessionNumberList></DataBank></DataBankList><GrantList CompleteYN="Y"><Grant><GrantID>S06 GM008016</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2006</Year><Month>01</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Hazard Mater</MedlineTA><NlmUniqueID>9422688</NlmUniqueID><ISSNLinking>0304-3894</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>4R7X1O2820</RegistryNumber><NameOfSubstance UI="D002713">Chlorine</NameOfSubstance></Chemical><Chemical><RegistryNumber>DFC2HB4I0K</RegistryNumber><NameOfSubstance UI="D011078">Polychlorinated Biphenyls</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.7.99.4</RegistryNumber><NameOfSubstance UI="D050901">Nitrate Reductase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002713" MajorTopicYN="N">Chlorine</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002851" MajorTopicYN="N">Chromatography, High Pressure Liquid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015971" MajorTopicYN="N">Gene Expression Regulation, Enzymologic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015966" MajorTopicYN="N">Gene Expression Regulation, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D050901" MajorTopicYN="N">Nitrate Reductase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011078" MajorTopicYN="N">Polychlorinated Biphenyls</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2005</Year><Month>06</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2005</Year><Month>11</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2005</Year><Month>11</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>1</Month><Day>13</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>10</Month><Day>27</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>1</Month><Day>13</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16406292</ArticleId><ArticleId IdType="pii">S0304-3894(05)00787-9</ArticleId><ArticleId IdType="doi">10.1016/j.jhazmat.2005.11.073</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>District de Columbia</li></region></list><tree><noCountry><name sortKey="Dutta, Sisir K" sort="Dutta, Sisir K" uniqKey="Dutta S" first="Sisir K" last="Dutta">Sisir K. Dutta</name><name sortKey="Perkins, Michael" sort="Perkins, Michael" uniqKey="Perkins M" first="Michael" last="Perkins">Michael Perkins</name></noCountry><country name="États-Unis"><region name="District de Columbia"><name sortKey="De, Supriyo" sort="De, Supriyo" uniqKey="De S" first="Supriyo" last="De">Supriyo De</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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